Determination of rabies-specific antibodies in wildlife sera.
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Determination of rabies-specific antibodies in wildlife sera.

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Published .
Written in English

Book details:

The Physical Object
Pagination109 leaves
Number of Pages109
ID Numbers
Open LibraryOL21797433M

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  Fig. 1a presents the distribution of the titres obtained with the BioPro Rabies ELISA Ab kit and the FAVN test on sera from unvaccinated animals and sera from vaccinated pets. Fig. 1b highlights the area around the threshold of IU/mL in Fig. 1a.. Download: Download full-size image Fig. 1. (a) Antibody titres obtained with the BioPro Rabies ELISA Ab kit and the FAVN test on the Cited by:   The determination of rabies neutralising antibodies in the sera of animals sampled in vaccinated areas is a reliable indicator of the vaccination’s effectiveness [ 2, 37 ].Cited by: 1.   Above all, the incubation period did not affect the neutralization of the virus. The test was also applied to the detection of rabies-specific antibody levels in 73 human sera. The test was found to be simple, quick and economical for titration of rabies antibodies. Introduction In India, rabies is a Cited by: 1. Several assays exist for the serological determination of antibody protection against rabies virus infection. of rabies-specific antibody in the sera of the major domestic and wildlife.

We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services.   The RFFIT was performed according to the technique described by Smith et al. to determine anti-rabies neutralizing antibody level in human and animal sera. In brief, serial five-fold dilutions of test sera were made and incubated with a constant amount of challenge virus-CVS 11 (50 FFD 50 / ml). The serial dilutions of SRIG (2 IU/ml) were used as a positive control reference and . The specific rabies antibodies in the monitoring of fox ORV campaigns can be detected by using two different methods: virus neutralisation test and ELISA. ELISA is simpler and faster method but the virus neutralization test is still considered as a golden standard. 1. Case definition. An animal is determined to be rabid after diagnosis by a qualified laboratory as specified (see Part I. A. Rabies diagnosis). The national case definition for animal rabies requires laboratory confirmation on the basis of either a positive result for the direct fluorescent antibody test (preferably performed on CNS tissue) or isolation of rabies virus in cell culture or.

  Several test methods are available for detecting rabies-specific antibodies. The first developed was the mouse neutralisation test (MNT). In this method, sera at different concentrations are mixed with a constant viral dose and are then inoculated into weanling mice. The mice are then observed for development of clinical symptoms.   Similarly an indirect ELISA that utilizes anti-canine immunoglobulin for the measurement of rabies antibodies in the sera of dogs and related species was developed by Barton and Campbell (), while Mebatsion et al., used protein A linked with horseradish peroxidase for detecting IgG antibody against rabies virus in the sera of humans and carnivores. Journal of Wildlife Diseases, 31(3) Age determination in bats. of rabies specific antibodies in the Mexican free-tailed bat (Tadarida brasiliensis mexicana) at. A rabies antibody titer is essentially an estimation of an immune response against rabies virus (either through exposure or vaccination). The RFFIT is one method which provides a laboratory measurement of the ability of an individual human or animal serum sample to neutralize rabies virus. There is no “protective” titer against rabies virus.